Neural Noise- TraSE-IN (Scientific Reports, 2024) is an open-source Python pipeline for Tracking and Spike Estimation of Individual Neurons in behaving Hydra vulgaris from dual-label (nuclear tdTomato + cytoplasmic GCaMP7s) imaging.1
- Modules segment and track each nucleus over time, extract GCaMP calcium activity, and predict individual spikes—targeting single-cell resolution neural activity during behavior.1
- The authors frame the work as a step toward recording every spike from every neuron in a behaving animal and toward whole-animal neural-code datasets.1